MBMB 451A Section Three - Fall 2004
Provisional SyllabusP. M. D. Hardwicke, Room 210W Neckers "C" Wing. Tel. 618-453-6469
This part of the course will involve some quantitative problems. Students are expected to have a background in organic and physical chemistry.
Lectures 1 - 2: Outline of Principles of Thermodynamics. Isolated, closed and open systems. Extensive and intensive quantities. First Law. Second Law, microstates, heat and entropy. DH, DS and DG. Criteria of equilibrium. The Gibbs equation (DG = DH - TDS). In spontaneous (natural) processes in closed systems DGT,P ≤ 0. Relation of DGT,P to net work. Activity (ai). Chemical potential (mi). Thermodynamic equilibrium constant. DGo, DG = DGo+ RT ln [P]/[R], DGo= - RT ln Keq, DG = - RT ln Keq/Q.
Effect of pH and reactant/product water on DG: DG' and DGo'. The changes in an extensive thermodynamic quantity in a consecutive series of processes can be summed (e.g., DGTOT = SDGi, KTOT = PKi.) DG of cycles. Elementary kinetics. Law of mass action. Equilibrium constants derived from law of mass action.
Chapter 3 (Voet & Voet)
Lecture 3 - 4: Ligand Binding. Dissociation and association constants. ‘Affinity’. ‘Saturation’. Important non-covalent forces in ligand and substrate binding and the folding of macromolecular. Simple ligand binding. Cooperative ligand binding. Agonists and antagonists.
Chapters 2 and 10 (Voet & Voet)
Lecture 4-5: Water. Autoprotonation of water. pH. Strong acids, strong bases, weak acids, weak bases. Henderson-Hasselbach equation. Effect of H2O and H+ on DG.
Chapter 2 (Voet & Voet)
Lecture 6-7: Amino acids. Isomers. Peptides. Isoelectric points (pI). Proteins. Forces involved in formation of protein structure. Primary, secondary, tertiary, and quaternary structure. Common motifs.
Chapter 8, and Chapter 9 sections1, 2, 3 and 4 (Voet & Voet)
Lecture 8: Myoglobin and hemoglobin as examples of ligand binding phenomena.
Chapter 10 (Voet & Voet)
Last updated on September 03, 2004.