The Division of Laboratory Animal Medicine has the capability of performing on-site blood analysis. The VetScan HM2 by Abaxis is a fully automated instrument which performs complete blood counts (CBC’s). The CBC measures 18 different parameters, including counts for: white blood cells, red blood cells, and platelets. Unlike many hematology systems, the HM2 can test a variety of species – including mice, rats, and guinea pigs. Only 75ul of EDTA preserved whole blood are needed for each sample. Results are printed out on 8.5”x11” paper. If sent to an outside lab, prices range from $35 to $50, not including shipping charges and supplies. The fee is $12.50 per sample, with a volume discount of $1 off per sample if 20 or more samples of the same strain are run at once from the same PI. Submission forms are located on the DLAM home page and are required with each batch. Please email completed submission forms to Andrea Frazier one week in advance of submitting samples.
MOBILE ANESTHESIA SYSTEM
The Rodent Surgery Setup is a mobile anesthesia system for mice and rats. It is available for rent at an hourly charge of $11.50. The RC2 Rodent Anesthesia System by VetEquip has the capability of running up to 6 animals at once. The rental fee includes a supply of oxygen, isoflurane, a hot bead sterilizer, a heated platform, and a large magnification light. The Rodent Surgery Setup is available for use in the procedure rooms in DLAM or in your lab. Please contact Andrea Frazier by email two days in advance to reserve this equipment.
ETHYLENE OXIDE STERILIZER
The AN74i by Anderson Products is a tabletop Ethylene Oxide Sterilizer. Ethylene oxide can be used to sterilize sensitive equipment that could not otherwise by adequately sterilized. Instruments frequently sterilized by EtO include: plastic instruments, rubber and plastic tubing, electrical equipment, surgical staplers/staples, and anesthesia masks and circuits. Items should be wrapped, ready to sterilize, and labeled when submitted. In addition to the 12 hour run time, most items need to aerate for a minimum of 24 hours before they are ready to use. Items will need to be submitted at least 2 days before they are needed for use. Please contact Andrea Frazier for more information.
The Faxitron is a self-contained X-ray unit. The Faxitron can be used for mice, rats, and guinea pigs. These digital images can then be transferred to a USB or a zip drive. A current, approved RCC application, LACUC protocol, and Cabinet X-ray Radiation Safety Training are required. Please contact Dr. Liberati for more information on use. Contact Rob Deters in the Office of Radiological Control for information on training.
ADDITIONAL EQUIPMENT LOCATED IN DLAM
The Rad Source Technologies RS 2000 Biological Research X-ray Irradiator is designed specifically as a non-isotope replacement for cesium (Cs-137) irradiators being used for the irradiation of small animals, cells, tissue, and other research purposes. The RS 2000 is a self-contained unit that provides 160kV operation at 25mA and uses a simple touch screen for easy programming. The dose rate delivered to an animal cage is ~110 rads/min (110cGy/min), and the dose uniformity exceeds 95%. Due to the dose uniformity, animals are free to move about in the animal cage during irradiation. For cell and tissue work, a movable shelf allows for dose rate adjustment from 800 to 1,200 rads/min (8-12 Gy/min) using unfiltered radiation. Typically for well plates and similar items, the average dose rate is 1,000 rads/min (10 Gy/min) with a 20.5 cm diameter field. A current, approved RCC application, LACUC protocol, and Cabinet X-ray Radiation Safety Training are required. Contact Rob Deters in the Office of Radiological Control for more information.
XENOGEN - IVIS LUMINA
The IVIS(r) Lumina Series II brings together leading optical imaging technologies into one easy to use and highly sensitive bench-top system. The IVIS Lumina II is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped with multiple filter sets that can be used to image reporters that emit from green to near-infrared. Superior spectral unmixing can be achieved by Lumina II's optional high resolution short cut off filters. Interested persons should sign up for a training session with Melissa Roberts, Research Imaging Facility, 545-7186.