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Description for Grants

Summary Description for Grants (Word Document)

SIU Core Laboratory
Flow Cytometry Facility

801 N. Rutledge Street
Room 2364
Springfield, Il 62794-9616
Phone: (217) 545-5812

Flow Cytometry Facility

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The Flow Cytometry Facility is a fully equipped high speed cell sorting and cell analyzing research laboratory. The facility is equipped with three flow cytometry instruments.

  • The Becton-Dickinson FACSAria II high-speed cell sorter, features five air cooled lasers (blue 488nm, yellow-green 561 nm, red 640nm, violet 405nm and UV355nm), multicolor analysis up to 10 parameters and four way sorting. This instrument is housed in a Baker BioProtect-II bio-safety cabinet, which enables sorting of BSL-2 level samples. FACSDiva software is used for acquisition and analysis.
  • The Becton-Dickinson FACSCalibur benchtop flow cytometer features two lasers (blue 488nm and red 635nm) with four color capability. CellQuest software is used for acquisition and analysis.
  • The Becton-Dickinson Accuri C6 bench top trained user operated flow cytometer features two lasers (blue 488nm and red 640nm) with four color capability. Luminex-BioPlex Array Reader is a flexible analyzer based on the principles of flow cytometry. The system enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays.