| |
|
|
|
| Research
Profile - Lydia A. Arbogast |
|
|
|
|
| Department/Division: |
|
|
Email
Address: |
| Physiology |
larbogast@siumed.edu |
| Office
Location: |
|
|
Location
of Laboratory: |
Life
Science III, Rm 2077
Carbondale |
|
Life
Science III, Rms 2015, 2025 Carbondale |
| Office
Phone Number: |
|
|
Laboratory
Phone Number: |
| 453-1856 |
|
|
453-8443 |
| Willing
to mentor medical students and / or residents?
|
Yes |
| Research
Interests: |
Neuroendocrinology,
Reproductive Biology, Endocrinology, Catecholamines,
Tyrosine Hydroxylase, Prolactin, Ovarian Steroids, Estradiol,
Progesterone, Lactation, Hypothalamus,Phosphorylation,
Gene Expression, Cell Signaling, Opioid Peptides |
| Techniques
and Methodologies: |
RT-PCR, In situ hybridization, Ribonuclease protection
assay, Plasmid Preparation, DNA cloning, Luciferase
Reporter Assays, High Performance Liquid Chromatography
for Catecholamines, Western Blot, Immunocytochemistry,
Primary Neuronal Cultures, Neuronal Cell Line Cultures,
Transfections, Radioimmunoassays, Confocal Microscopy,
Phosphate Incorporation, Animal Surgery, Brain microdissection,
Protein, DNA and RNA electrophoresis |
|
Equipment:
|
Cell
culture hood and incubators, Microscopes, Cryostat,
Centrifuges, Water Baths, Spectrophotometer, Incubators,
Confocal Laser Scanning Microscope (in Rm 2051) |
Current
Collaborators:
|
|
Current
and Pending Awards:
Current
Prolactin Feedback to Hypothalamic Dopaminergic Neurons,
PI, NIH
Opioid-Dopamine Interactions during Lactation, PI, NIH |
| Publications:
|
|
|
|
Arbogast
LA, Voogt JL. Prolactin (PRL) receptors are colocalized
in dopaminergic neurons in fetal hypothalamic cell
cultures: Effect of PRL on tyrosine hydroxylase activity.
Endocrinology 138:3016-3023, 1997
Arbogast LA, Voogt JL. Endogenous opioid peptides
contribute to suckling-induced prolactin release by
suppressing tyrosine hydroxylase activity and mRNA
levels in the tuberoinfundibular dopaminergic neurons.
Endocrinology 139: 2857-2862, 1998
Arbogast LA, Shah GV, Voogt JL. 3‚,5‚
Cyclic adenosine monophosphate mediates the salmon
calcitonin-induced increase in hypothalamic tyrosine
hydroxylase activity. Endocrinology 140:3273-3281,
1999
Arbogast LA, Hyde, JF. Estradiol attenuates the forskolin-induced
increase in hypothalamic tyrosine hydroxylase activity.
Neuroendocrinology 71:219-227,2000
Arbogast LA. Calmodulin and a cyclic nucleotide protein
kinase facilitate the prolactin-induced increase in
tyrosine hydroxylase activity in tuberoinfundibular
dopaminergic neurons Endocrine 16:105-112, 2001
Arbogast LA, Voogt JL. Progesterone induces dephosphorylation
and inactivation of tyrosine hydroxylase in hypothalamic
dopaminergic neurons. Neuroendocrinology 75:273-281,
2002
Nahi F, Arbogast LA. Prolactin modulates hypothalamic
preproenkephalin, but not proopiomelanocortin, gene
expression during lactation. Endocrine 20:115-122,
2003 |
Optional-CV
Web address:
|
|
| |
|
|
| |
|
|
|
|
|
|
|
