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| Research Profile - Michael
Collard |
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| Department/Division: |
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Email
Address: |
| Physiology |
mcollard@siumed.edu |
| Office
Location: |
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Location
of Laboratory: |
| Life
Science III, Room 2081, Carbondale |
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Life Science III, Room 2018, 2040 |
| Office
Phone Number: |
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Laboratory
Phone Number: |
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618-453-8430 |
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618-453-1592 |
| Willing
to mentor medical students and / or residents?
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Yes |
| Research
Interests: |
Cancer,
epigenetics, mouse
knockouts, testis, gene expression,
signal transduction, tumor
suppressors, genomic imprinting,
fetal development |
| Techniques
and Methodologies: |
rat
and mouse animal models, cell
culture, recombinant protein
production, bacterial and eukaryotic
gene expression, site directed
mutagenesis, fluorescence
microscopy, microarray analysis,
bioinformatics, general molecular
biology |
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Equipment:
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Cell
culture equipment, confocal
microscopy |
Current
Collaborators:
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Jodi
Huggenvik, Ko Watabe, Tom
Tarter, Carl Faingold, Earl Parr, Stan
McKnight (University of Washington) |
| Current
and Pending Awards: |
Current:
2001-2006 Co-Principal Investigator: NUDR: A Potential
Tumor Suppressor of Childhood Cancers, NIH grant R1CA89438A
( J.I. Huggenvik, P.I.)
2003-2005 Co-Principal Investigator: Tumor Suppressor
Activities of NUDR and PTEN in Prostate. Activities
to Promote Research Collaborations (NIH/NCI supplement)
( J.I. Huggenvik, P.I.)
SIU Cancer Institute Grant "The Role of DNA Repair
Enzymes in Prostate Cancer". M.W. Collard, Principal
Investigator
Pending:
Co-investigator: Genetics and Preservation of an Endangered
Rat
Resource: NIH R01 proposal submitted 6-01-03 (total
cost requested, Carl L. Faingold, P.I.) |
| Publications:
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Collard,
M.W. , Griswold, M.D. (1987) Biosynthesis and molecular
cloning of sulfated glycoprotein 2 secreted by rat
Sertoli cells. Biochemistry 26:3297-3303.
Collard, M.W., Sylvester, S.R., Tsuruta, J.K., Griswold,
M.D. (1988) Biosynthesis and molecular cloning of
sulfated glycoprotein 1 secreted by rat Sertoli cells:
Sequence similarity with the 70-Kilodalton precursor
to sulfatide/GM1 activator. Biochemistry 27:4557-4564.
Cairns, B.R., Collard, M.W., Landfear, S.M. (1989)
Developmentally regulated gene from Leishmania encodes
a putative membrane transport protein. Proc. Natl.
Acad. Sci. USA 86:7682-7686.
Collard, M.W., Day, R., Akil, H., Uhler, M.D., Douglass,
J.O. (1990) Sertoli cells are the primary site of
prodynorphin gene expression in rat testis: Regulation
of mRNA and secreted peptide levels by cAMP analogs
in cultured cells. Molecular Endocrinology 4:1488-1496.
Day, R., Schafer, M.K.H., Collard, M.W., Watson, S.J.,
Akil, H. (1991) Atypical prodynorphin gene expression
in corticosteroid producing cells of the rat adrenal
gland. Proc. Natl. Acad. Sci. USA 88:1320-1324
Huggenvik J.I., Michelson R.J., Collard M.W., Ziemba
A.J., Gurley P., Mowen K.A. (1998) Characterization
of a Deformed Epidermal Autoregulatory Factor-1 (DEAF-1)-Related
(NUDR) Transcriptional Regulator Protein. Molecular
Endocrinology 12:1619-1639
Michelson R.J., Collard M.W., Ziemba A.J., Persinger
J., Bartholomew B., Huggenvik J.I. (1999) Nuclear
DEAF-1-related (NUDR) protein contains a novel DNA
binding domain and represses transcription of the
heterogeneous nuclear ribonuclearprotein A2/B1 promoter.
Journal of Biological Chemistry 274:30510-30519
Bottomley M.J., Collard M.W., Huggenvik J.I., Liu
Z., Gibson T.J., Sattler M. (2001) The SAND domain
structure defines a novel DNA-binding fold in transcriptional
regulation. Nature Structural Biology Jul;8(7):626-633
Jensik P.J., Holbird D., Collard M.W., Cox T.C., (2001)
Cloning and characterization of a functional P2X receptor
from larval bullfrog skin American Journal of Physiology
281:C954-962
Jensik PJ, Huggenvik JI, Collard MW (2004) Identification
of a nuclear export signal and protein interaction
domains in deformed epidermal autoregulatory factor-1
(DEAF-1).
J Biol Chem. 2004 May 25 [Epub
ahead of print] LINK
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Optional-CV
Web address:
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http://131.230.175.67/
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