Research at SIU SOM
 
 
Research Profile - Peter Hardwicke  
   
Department/Division:     Email Address:
Biochemistry and Molecular Biology phardwicke@siumed.edu
Office Location:     Location of Laboratory:
Room 210W Neckers "C" WING, Carbondale   Room 204 Neckers "C" Wing
Office Phone Number:     Laboratory Phone Number:
618-453-6469     618-453-6403
Willing to mentor medical students and / or residents? Yes
Research Interests: Ca2+ transport by the Ca2+-ATPase and the Na+-Ca2+ exchanger
Regulation of Ca2+ levels in cells
Conjugated trienes in excitable tissues
Membrane proteolipids
Role of plasmacytoid cells in multiple sclerosis
Techniques and Methodologies: Molecular biology
Immunology
Fluorescence
NMR
CD
Electron microscopy
Protein chemistry

Equipment:

Lambda 40 sprectophotometer
SLM 8000c Spectrophotofluorimeter
Current Collaborators:
Kenneth Philipson (UCLA, sodium calcium exhanger)
David Stokes (Skirball Institute, Ca2+-ATPase)
Current and Pending Awards:
 
Publications:    

Kalabokis, V., Bozzola, J. J., Castellani, L., and Hardwicke, P. M. D. (1991) "A Possible Role for the Dimer Ribbon State of Scallop Sarcoplasmic Reticulum: Dimer Ribbons Are Associated with Stabilization of the Ca2+-Free Ca-ATPase".J. Biol. Chem. 266, 22044-22050

Kalabokis, V. N. and Hardwicke, P. M. D. (1993) "Effect of the Biochemical State of the Ca-ATPase Protein of Scallop Sarcoplasmic Reticulum on Its Interaction with Trans-parinaric Acid" Biochim. Biophys. Acta, 1147, 35-41.

Kalabokis, V., Santoro, M. M. and Hardwicke, P. M. D. (1993) "Effect of Na+ and Nucleotide on the Stability of Solubilized Ca2+-Free Ca-ATPase from Scallop Sarcoplasmic Reticulum." Biochemistry , 32, 4389-4396.

Shi, X., Chen, M., Huvos, P. E. and Hardwicke, P. M. D. (1998) "Amino Acid Sequence of a Ca2+-transporting ATPase from the Sarcoplasmic Reticulum of the Cross-striated Part of the Adductor Muscle of the Deep Sea Scallop: Comparison to SERCA Enzymes of Other Animals." Comp. Biochem. Physiol., Part B, 120, 359-374

Hardwicke, P. M. D., Ryan, C. and Kalabokis, V. N. (1999) "A Novel Small Protein Associated with a Conjugated Trienoic Chromophore from Membranes of Scallop Adductor Muscle. Phosphorylation by Protein Kinase A" Biochim. Biophys. Acta, 1417, 1-8

Chen, M., Zhang, Z., Tawiah-Boateng, M. -A. and Hardwicke, P. M. D. (2000) "A Ca2+-dependent Tryptic Cleavage Site and a Protein Kinase A Phosphorylation Site are Present in the Ca2+ Regulatory Domain of Scallop Muscle Na+-Ca2+ Exchanger" J. Biol. Chem., 275, 22961-22968

Ryan, C., Stokes, D. L., Chen, M., Zhang, Z. and Hardwicke, P. M. D. (2004) "Effect of Orthophosphate, Nucleotide Analogues, ADP, and Phosphorylation on the Cytoplasmic Domains of Ca2+-ATPase from Scallop Sarcoplasmic Reticulum" J. Biol. Chem., 279, 5380-5386

Crawford, C. L. and Hardwicke, P. M. D. (2003) "Animal Models of Human Diabetic Polyneuropathy" JAMA, 289, 1779-1780

Crawford, C. L. and Hardwicke, P. M. (2004) "Plasma or Plasmacytoid Cells in Multiple Sclerosis?" J. Clin. Invest., 113, 794

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