Profile - Narayan, Prema

Department/Division:			Email Address:
Physiology			pnarayan@siumed.edu
Office Location:			Location of Laboratory:
2070, LS III - Carbondale			2031, LS III
Office Phone Number:			Laboratory Phone Number:
618-453-1567			618-453-1545
Willing to mentor medical students and / or residents?		Yes
Research Interests:	Reproductive endocrinology, constitutively activating mutations, gonadotropin receptor action, signaling pathways in gonadal pathophysiology
Techniques and Methodologies:	Transgenic and knock-in mice, cloning, gene expression studies using real-time RT-PCR, Western blot analysis, immunohistochemistry, in- situ hybridization, hormone assays, proteomic analysis in reproductive tissues
Equipment:	Bio-Rad real time RT-PCR machine, Bior-Rad protean IEF system, Leica dissecting microscopes, Leica digital microscope with brightfield, phase, DIC and fluorescence capabilities and Q-imaging pro software, UVP imaging workstation for DNA and protein gels, standard electrophoresis equipment for DNA and protein gels.
Current Collaborators:
Current and Pending Awards: Current: NIH ROI titled " Constitutively active LH receptors in transgenic mice"

Publications:
1. Narayan, P., Gray, J. and Puett, D. (2000) A biologically active single chain human chorionic gonadotropin analog with altered receptor binding properties. Endocrinology 141: 67-71. 2. Angelova, K., Narayan, P., Simon, J.P. and Puett, D. (2000) Functional role of transmembrane helix 7 in the activation of the heptahelical lutropin receptor. Mol. Endocrinol. 14: 459-471. 3. Fralish, G.B., Narayan, P. and Puett, D. (2001) High-level expression of a functional single chain hCG-LH receptor ectodomain complex in insect cells. Endocrinology 142:1517-152. 4. Narayan, P., Gray, J. and Puett, D. (2002) Yoked complexes of human choriogonadotropin and the lutropin receptor: evidence that monomeric individual subunits are inactive. Mol Endocrinol. 16: 2733-2745. 5. Schubert, R.L., Narayan, P. and Puett, D. (2003) Specificity of cognate ligand-receptor interactions: fusion proteins of human chorionic gonadotropin and the heptahelical receptors for human luteinizing hormone, thyroid-stimulating hormone, and follicle-stimulating hormone. Endocrinology 144: 129-137. 6. Fralish, G.B., Narayan, P. and Puett, D. (2003) Consequences of single-chain translation on the structures of two chorionic gonadotropin yoked analogs in the ?-? and ?-? configurations. Mol. Endocrinol. 17: 757-767. 7. Meehan, T.P., Puett, D. and Narayan, P. (2004) Tightly regulated and inducible expression of a yoked hormone-receptor complex in HEK 293 cells. J. Mol. Endocrinol. 32: 247-255. 8. Angelova, K., Fremont, V., Jain, R., Zhang, M., Puett, D., Narayan, P. and Szkudlinski, M.W. (2004) Human ?-subunit analogs act as partial agonists to the thyroid stimulating hormone receptor: Differential effects of free and yoked subunits. Endocrine 24: 25-32. 9. Meehan, T.P., Harmon, B.G., Overcast, M.E., Yu, K.K., Camper, S.A., Puett, D. and Narayan, P. (2005) Gonadal defects and hormonal alterations in transgenic mice expressing a single chain human chorionic gonadotropin-lutropin receptor complex. J. Mol. Endocrinol 34:489-503. 10. Meehan, T.P. and Narayan, P. (2006) Constitutively active luteinizing hormone receptors: consequences of in vivo expression. Mol, Cell. Endocrinol. (in press).
Optional-CV Web address: