Rybak, Leonard P.

Department/Division:			Email Address:
Surgery/Otolaryngology			lrybak@siumed.edu
Office Location:			Location of Laboratory:
747 N. Rutledge (Baylis Bldg)			4th floor 801 N. Rutledge
Office Phone Number:			Laboratory Phone Number:
217-545-2598			217-545-0955
Willing to mentor medical students and / or residents?		Yes
Research Interests:	ototoxicity mechanisms (cisplatin) and prevention noise-induced hearing loss auditory development cell migration in the cochlea transplantation of cochlear cells, adenosine receptors in the cochlea and kidney
Techniques and Methodologies:	cochlear hair cell culture immunohistochemistry, RT/PCR auditory brainstem response, endocochlear potential, scanning and transmission electron microscopy, image analysis, TUNEL analysis
Equipment:	auditory stimulus and recording equipment (Tucker Davis) electrometer, HPLC, microelectrodes
Current Collaborators:	Craig Whitworth (lab director) Vickram Ramkumar (co-investigator) George Dunaway (co-investigator)
Current and Pending Awards:
Current: NIH (NIDCD) Endogenous Modulation of Cochlear Injury (April 2, 2004-March 31, 2007)(PI); Repair of cochlear injury by transplantation, American Otological Society, July 1, 2004 to June 30, 2005 (Co-PI)
Publications:
Ramkumar V, Ravi R, Wilson MC, Gettys TW, Whitworth C, Rybak LP: Identification of A1 adenosine receptors in rat cochlea coupled to inhibition of adenylate cyclase. Am J Physiol 267:C731-7, 1994. Ford MS, Maggirwar SB, Rybak LP, Whitworth C, Ramkumar, V: Expreession and function of adenosine receptors in the chinchilla cochlea. Hear Res 105:130-140, 1997. Ford MS, Nie Z, Whitworth C, Rybak LP, Ramkumar V: Up-regulation of adenosine receptors in the cochlea by cisplatin. Hear Res 111:143-152, 1997. Nie Z, Mei Y, Ford M, Rybak L, Marcuzzi A, Ren H, Stiles GL, Ramkumar V.: Oxidative stress increases A1 adenosine receptors expressison by activating nuclear factor kB. Mol Pharmacol 53:663-669, 1998. Meech RP, Campbell KCM, Hughes LP, Rybak LP: A semiquantitave analysis of the effect of cisplatin on the rat stria vascularis. Hear Res 124:44-59, 1998. Campbell KCM, Meech RP, Rybak LP, Hughes LP: D-methionine protects against cisplatin damage to the stria vascularis. Hear Res 138:13-28, 1999. Kamimura T, Whitworth CA, Rybak LP: Effect of 4-methylthiobenzoic acid on cisplatin-induced ototoxicity in the rat. Hear Res 131:117-127, 1999. Bhat SG, Mishra S, Mei Y, Nie, Z, Whitworth CA, Rybak LP, Ramkumar V: Cisplatin up-regulates the adenosine A1 receptor in the rat kidney. European J Pharmacol 442:251-264, 2002. Whitworth CA, Ramkumar V, Jones B, Tsukasaki N, Rybak LP: Protection against cisplatin ototoxicity by adenosine agonists. Biochem Pharmacol 67:1801-1807, 2004. Pingle SC, Mishra S, Marcuzzi A, Bhat SG, Sekino Y, Rybak LP, Ramkumar V: Osmotic diuretics induce adenosine A1 receptor expression and protect renal proximal tubular cells against cisplatin-mediated apoptosis. J Biol Chem (in press)(published on-line July 22, 2004 @jbc.org)
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