Tissue Banking Facility
The Tissue Banking Facility (TBF) is a service provided by the Simmons Cancer Institute at SIU to obtain surgically resected tissue while safeguarding all aspects of patient care. The Springfield Committee for Research Involving Human Subjects (SCRIHS) authorizes our TB Facility to obtain tissue for research that would otherwise be resected for medical purposes and discarded.
This facility offers several advantages over other national tissue repositories. They include:
- Tissue can be handled by sterile techniques if requested by the investigator.
- Tissue is processed immediately and can be delivered fresh.
- Tissue is processed according to researcher specifications.
- The location of the TBF at SIU is convenient.
- Specimens may be picked up by the investigator from the TBF to avoid shipping charges.
- Pathologists from Memorial Medical Center and St. John’s Hospital assist in the tissue collection.
- Surgical pathology reports are provided for each specimen.
Skilled technologists work with surgeons and pathologists to maximize the scientific value of the acquired tissue without compromising diagnostic processing. In addition, the TBF provides investigators access to histopathologic parameters available from the final diagnostic report while preserving individual patient confidentiality.
Tissues may be obtained fresh (including sterile for establishment of cell culture), frozen in liquid nitrogen, embedded in the cryopreservative O.C.T, or fixed in formalin and paraffin embedded.
Who Can USE TBF Services?
Services of the Simmons Cancer Institute Tissue Bank Facility are available to research investigators of Southern Illinois University. To obtain tissue from the TBF, investigators are required to sign a user agreement and file an application form with the TBF. The Tissue Bank Committee prioritizes tissue allocation based on the research purposes and funding of the project and the availability of tissue. Tissue obtained from the TBF may not be distributed to third parties.
The TBF technician will work with each investigator to maximize the value of the tissue for a particular scientific study.
Laboratory Space and Equipment
Most of the tissue collection done by the TBF staff takes place in the Surgical Pathology Gross room at Memorial Medical Center or St. John’s Hospital. These locations are ideal for the rapid retrieval of tissue immediately following resection from the patients. Following a diagnosis by the surgical pathologist, TBF staff members process the tissue using equipment located in this room. A light microscope is used to examine the tissues for quality control.
The TBF freezer is located in the 911 Building. Tissue samples are stored in the vapor phase in a liquid nitrogen storage freezer equipped with an alarm. Rental of storage space in the freezer is available on a monthly basis. Samples of sera and other body fluids are stored at –80°C equipped with an alarm. Cryostat—A cryostat is available for researchers to use to cut sections of their frozen tissues. Training is mandatory and must be documented by TBF personnel prior to the use of this machine. Alternatively, TBF staff can cut sections of your frozen tissues for you (charges are assessed on an hourly basis or per slide). Microtome—A microtome is available for researchers to use to cut paraffin–embedded sections of their tissues. Training is mandatory and must be documented by TBF personnel prior to the use of this machine. Alternatively, TBF staff can cut sections of your paraffin-embedded tissues for you (charges are assessed on an hourly basis or per slide).