The work in this lab is concerned with the physiology, biochemistry, and genetics of chitin biosynthesis in the filamentous fungus Aspergillus nidulans. Chitin is the most important structural polymer in the cell wall of the fungi and it is believed to be functionally analogous to peptidoglycan in the bacteria. We are concerned with the regulation of chitin biosynthesis both temporally and spatially. We have cloned and intend to sequence several genes involved in chitin synthesis and are characterizing the role of the gene products.
Borgia, P.T., Y. Miao, and C.L. Dodge. 1996. The orlA gene from Aspergillus nidulans, homologous to the TPS2 genefrom Sacchoarmyces cerevisiae, is necessary for normal chitin synthesis and growth at 42. Mol. Microbiol. 20:1287-1296.
Borgia, P.T., N. Iartchouk, P.J. Riggle, K.R. Winter, Y. Koltin, and C. Bulawa. 1996. The chsB gene from Aspergillus nidulans is necessary for normal hyphal growth and development, Fungal Genetics and Biology. 20:193-203.
Specht, C.A., Y. Liu, P.W. Robbins, C.E. Bulawa, N. Iartchouk, K.R. Winter, P.J. Riggle, J.C. Rhodes, C.L. Dodge, D.W. Culp, and P.T. Borgia. 1996. The chsD and chsE genes of Aspergillus nidulans and their roles in chitin synthesis, Fungal Genetics and Biology. 20:153-167
C. Lawyer, S. Pai, M. Watabe, P. Borgia, T. Mashimo, L. Eagleton and K. Watabe. 1996. Antimicrobial activity of a 13 amino acid tryptophan-rich peptide derived froma putative porcine precursor protein of a novel family of antibacterial peptides. FEBS Lett. 390:95-9