Elucidation of the cellular and molecular mechanisms controlling proliferation and differentiation of epithelial cells and determine how defects in such mechanisms could lead to malignant transformation. Focus is on 1), Ligand/receptor function and its intracellular signal transduction mechanisms; and 2), cellular adhesions such as cell-cell and cell-matrix adhesions. Chemoprevention of cancer and cancer biomarkers is another focus of this laboratory.
Wang, H., Radjendirane, V., Wary, K.K., and Chakrabarty, S . Transforming growth factor b regulates cell-cell adhesion through extracellular matrix remodeling and activation of focal adhesion kinase in human colon carcinoma Moser cells. Oncogene, 23, 5558-5561, 2004.
Chakrabarty, S., and Wang, H. Cellular adhesion and control of colon epithelial cell growth and differentiation.: Role of transforming growth factor b and the calcium sensing receptor. J. Clin. Ligand Assay. 27: no.2 , 104-111, 2004.
Wang, H., and Chakrabarty, S. Lipids and lipid-like molecules in cellular proliferation and differentiation, potential in cancer chemoprevention and treatment. J. Clin. Ligand Assay. 27, no. 2, 118-132, 2004.
Chakrabarty, S., Wang, H., Canaff, L., Hendy, G.N., Appelman, H., and Varani, J. Calcium Sensing Receptor in Human Colon Carcinoma: Interaction with Ca 2+ and 1,25-dihydroxyvitamin D 3. Cancer Res., 65, 493-398, 2005.
Bghagavathula, N., Kelley, E.A., Reddy, M., Nerusu, K.C., Leonard, C., Fay, K., Chakrabarty, S., and Varani, J. Upregulation of calcium-sensing receptor and mitogen-activated protein kinase signaling in the regulation of growth and differentiation in colon carcinoma. Br. J. Cancer, 93, 1364-1371, 2005. Pub Med
Chakrabarty, S., Wang, H., Canaff, L., Hendy, G.N., Appelman, H., and Varani, J. Calcium sensing receptor in human colon carcinoma: interaction with Ca2+ and 1,25-dihydroxyvitamin D3. Cancer Res., 65, 493-398, 2005.
Wang, H., Rajan, S., Liu, G., and Chakrabarty, S. Transforming growth factor β suppresses β-catenin/wnt signaling and stimulates an adhesion response in human colon carcinoma cells in a Smad4/DPC4 independent manner. Cancer Letts., 264, 281-287, 2008.
Wang, H., Promkan, M., Liu, G., and Chakrabarty, S. Switch of transforming growth factor β function from tumor suppression to stimulation in adenomatous polyposis coli (APC) knocked-down human colon carcinoma cells. Cancer Letts., 272, 253-259, 2008.
Liu, G., Hu, X, Varani, J., and Chakrabarty, S. Calcium and calcium sensing receptor modulates the expression of thymidylate synthase, NAD(P)H:quinone oxidoreductase 1 and survivin in human colon carcinoma cells: promotion of cytotoxic response to mitomycin C and fluorouracil. Mol. Carcinogenesis, 48, 202–211, 2009.
Liu, G., Hu, X., and Chakrabarty, S. Calcium sensing receptor down-regulates malignant cell behavior and promotes chemosensitivity in human breast cancer cells. Cell Calcium, 45, 216–225, 2009.
Aslam, M.D., Bhagavathula, N., Hu, X., Chakrabarty, S., and Varani, J. Growth-inhibitory effects of Aquamin, a mineralized extract from the red algae, Lithothamnion calcareum, on Ca2+-sensitive and Ca2+-resistant human colon carcinoma cells. Cancer Letts., 283, 186-192, 2009.
Promkan, M., Liu, G., Patmasiriwat, P., and Chakrabarty, S. BRCA1 modulates malignant cell behavior, the expression of survivin and chemosensitivity in human breast cancer cells. International J Cancer, 125, 2820-2828, 2009.
Liu, G., Hu, X., and Chakrabarty, S. Vitamin D mediates its action in human colon carcinoma cells in a calcium-sensing receptor-dependent manner: downregulates malignant cell behavior and the expression of thymidylate synthase and survivin and promotes cellular sensitivity to 5-FU. International J Cancer, 126, 631-639, 2010.
Liu, G., Hu, X, Premkumar, L and Chakrabarty, S. Nifedipine Synergizes With Calcium in Activating the Calcium Sensing Receptor, Suppressing the Expression of Thymidylate Synthase and Survivin and Promoting Sensitivity to Fluorouracil in Human Colon Carcinoma Cells. Mol. Carcinogenesis, 50, 922-930, 2011.
Promkan, M., Liu, G., Patmasiriwat, P., and Chakrabarty, S. BRCA1 suppresses the expression of survivin and promotes sensitivity to paclitaxel through the calcium sensing receptor (CaSR) in human breast cancer cells. Cell Calcium, 49, 79-88,2011.