SIU School of Medicine

Dale Buchanan Hales, PhD


Journal Articles (Refereed Journals)

Betz, G., Tsai, P. and Hales, D. B. "Reconstitution of steroid 17,20-Lyase activity after separation and purification of cytochrome P-450 and its reductase from rat testis microsomes." Endocrinology 107 :1055-1060, 1980.

Betz, G., Miller, H. H. and Hales, D. B. "Actions of Danazol in vivo on cytochrome P-450 and steroidogenic enzymes in rat testis and liver microsomal preparations." American Journal of Obstetrics and Gynecology 141 :962-972, 1981.

Hales, D. B. , Betz, G. "Steroid interactions with the cytochrome P-450 from testis microsomes." Journal of Steroid Biochemistry 21 :639-642, 1984.

Hales, D. B. , Betz, G. "NADPH-cytochrome P-450 reductase from testis and liver microsomes of untreated rats: isolation and comparison." Endocrinology 119 :811-818, 1986.

Hales, D.B. , Sha, L., and Payne, A.H. "Testosterone inhibits cAMP-induced de novo synthesis of Leydig cell cytochrome P-450 17 a by an androgen receptor-mediated mechanism." Journal of Biological Chemistry 262 :11200-11206, 1987.

Hales, D.B. , Ho, B., and Thompson, J.A. "Inter- and intramolecular deuterium isotope effects on the cytochrome P-450-catalyzed oxidative dehalogenation of 1,1,2,2-tetrachloroethane." Biochem. Biophys. Res. Commun. 149 :319-325, 1987.

Hales, D.B. , and Payne, A.H. "Regulation of de novo synthesis of cytochrome P-450 17 a in mouse Leydig cell cultures." Annals of the New York Academy of Sciences , NY , 513 :387-390, 1987.

Hales, D.B. and Payne, A.H. "Regulation of P450 scc mRNA and de novo synthesis in cultured Leydig cells." Endocrinology 124 :2099-2104, 1989.

Hales, D.B. , Sha, L. and Payne, A.H. "Glucocorticoid and cAMP-mediated induction of cholesterol side-chain cleavage cytochrome P450 (P450 scc ) in MA-10 tumor Leydig cells. Increases in mRNA are cycloheximide sensitive". Endocrinology 126 :2800-2808, 1990.

Keyes, P.L., Kostyo, J.L., Hales, D.B. , Chou, S., Constantino, C.X., Payne, A.H. "The biosynthesis of cholesterol side-chain cleavage cytochrome P450 in the rabbit corpus luteum depends upon estrogen." Endocrinology 127 :1186-1193, 1990.

Durkee, T.J., McLean , M.P., Hales, D.B. , Payne, A.H., Waterman, M.R., Khan, I. , Gibori, G. "P450 17 a and P450 scc gene expression and regulation in the rat trophoblast." Endocrinology 130 :1309-1317, 1992.

Hales, D.B. , Xiong, Y., Tur-Kaspa, I. "The role of cytokines in the regulation of Leydig cell P450c17 gene expression." Journal of Steroid Biochemistry and Molecular Biology 43 :907-914, 1992.

Hales, D.B. "Interleukin-1 inhibits Leydig cell steroidogenesis primarily by decreasing 17 a -hydroxylase/C 17-20 lyase cytochrome P450 (P450c17) expression." Endocrinology 131 :2165-2172, 1992.

Xiong, Y., Hales, D.B. "The role of tumor necrosis factor- a in the regulation of mouse Leydig cell steroidogenesis." Endocrinology 132 :2438-2444, 1993.

Xiong, Y., Hales, D.B. "Expression, regulation and production of tumor necrosis factor- a in mouse testicular interstitial macrophages in vitro ." Endocrinology 133 :2568-2573, 1993

Xiong, Y., Hales, D.B. "Immune-endocrine interactions in the mouse testis: cytokine-mediated inhibition of Leydig cell steroidogenesis." Endocrine Journal 2 :223-228, 1994

 Li, X., Youngblood, G.L., Payne, A.H., Hales, D.B. "Tumor necrosis factor- a inhibition of 17 a -hydroxylase/C17-20 lyase ( Cyp17 ) expression." Endocrinology 136 :3519-3526, 1995

Naaman-Reperant, E., Hales, D.B ., Durand, P. "Effect of transforming growth factor beta-1 (TGF b 1) on the cholesterol side chain cleavage system in the adrenal gland of sheep fetuses and newborns." Endocrinology 137:886-892 (1996)

McLean , M.P., Warden, K.J., Irby, R.B., Hales, D.B. "Altered ovarian strerol carrier protein expression in the pregnant streptozotocin-treated diabetic rat." Biology of Reproduction 55 :38-46 (1996)

Sharma, A.C., Bosmann, H.B., Motew, S.J., Hales, K.H., Hales, D.B. , Ferguson , J.L. "Steroid hormone alterations following induction of chronic intraperitoneal sepsis in male rats." Shock 6 :150-154 (1996)

Bosmann, H.B., Held Hales, K., Li, X., Liu, Z., Stocco, D.M., Hales, D.B . "Acute-decrease in serum testosterone during endotoxic shock is due to inhibition of Steroidogenesis Acute Regulator (StAR) protein expression." Endocrinology 137 :4522-4525 (1996)

Li, X., Held Hales, K., Watanabe, G., Lee, R.J., Pestell, R.G., Hales, D.B . "cAMP and Tumor Necrosis Factor- a induce AP-1 activity through independent mechanisms in MA-10 Tumor Leydig Cells." Endocrine 6: 317-324 (1997)

Clark , B.J., Combs, R., Held Hales, K., Hales, D.B. , Stocco, D.M. "Inhibition of transcription affects the synthesis of the steroidogenic acute regulatory protein (StAR) and steroidogenesis in MA-10 mouse Leydig tumor cells." Endocrinology 138 :4893-4901(1997).

Xiong, Y., Hales, D.B ., "Differential effects of tumor necrosis factor- a and interleukin-1 on 3 b -hydroxy steroid dehydrogenase D 4 - D 5 isomerase expression in Leydig cells." Endocrine 7 :295-301 (1997)

Ronen-Fuhrmann, T., Timberg, R., King, S.R., Held Hales, K., Hales D.B ., Stocco, D.M., Orly , J., "Spatio-temporal expression patterns of steroidogenic acute regulatory protein (StAR) during follicular development in the rat ovary." Endocrinology 139 :303-315 (1998).

Hales, D.B ., Greene, R.H., "Arginine vasopressin inhibition of P450c17 and testosterone production in mouse Leydig cells" Endocrine 8 :19 -28 (1998)

Sharma, A.C., Sam, A.D., Lee, L.Y., Hales, D.B ., Law, W.R., Ferguson , J.L., Bosmann, H.B. "Nitric Oxide Synthase-mediated regulation of testicular blood flow and serum steroid hormone concentrations during chronic intraperitoneal sepsis." Shock 9 :416-421 (1998)

Shi, Q., Hales, D.B ., Emanuele, N.V., Emanuele, M.A. "Interaction of ethanol and nitric oxide in the hypothalamic-pituitary-gonadal axis in the male rat." Alcoholism: Clinical and Experimental Research 22 :1754-1762 (1998)

Sandoff, T.W., Hales, D.B. , Held Hales, K., McLean , M.P. "Transcriptional regulation of the rat Steroidogenic Acute Regulatory (StAR) Protein gene by Steroidogenic Factor-1." Endocrinology 139 :4820-4831 (1998)

Fleury, A., Ducharme, L., Hales, D.B. , Stocco, D.M., LeHoux, J.G., "Acute in vivo effects of ACTH on the expression of steroidogenic acute regulatory protein in rat adrenal . " Endocrine Research 24 : 571-4 (1998)

Oglivie, K.M., Held Hales, K., Roberts, M., Hales, D.B. , Rivier, C. "The inhibitory effects of intracerbroventricularly injected IL-1 b on testosterone secretion in the rat: role of Steroidogenic Acute Regulatory (StAR) Protein." Biology of Reproduction 60 527-533 (1999)

Sam, A.D. II, Sharma, A.C., Lee, L.Y., Hales, D.B. , Law, W.R., Ferguson, J.L., Bosmann, H.B. "Sepsis produces depression of testosterone and steroidogenic acute regulatory (StAR) protein." Shock 11 :298-301 (1999)

Hales D.B. , Diemer T., Held Hales K. "Review: The Role of Cytokines in Testicular Function" Endocrine 10 :201-217 (1999)

Fiedler, E.P., Plouffe, L., Hales, D.B. , Held Hales, K., Khan, I. "Prostaglandin F2 a induces a rapid decline in progesterone production and Steroidogenic Acute Regulatory Protein expression in isolated rat corpus luteum without altering transcription." Biology of Reproduction 61 :643-650 (1999).

Lehoux, J.G., Hales, D.B., Fleury, A., Briere, N., Martel, D., Ducharme, L. "The in vivo effects of adrenocorticotropin (ACTH) and sodium restriction on the formation of different species of Steroidogenic Acute Regulatory Protein (StAR) in rat adrenal." Endocrinology 140 :5154-5164 (1999).

Diemer, T., Ludwig, M., Huwe, P., Hales, D.B. , Weidner, W. "Influence of urogenital infection on sperm function." Current Opinions in Urology 10:39-44 (2000).

Ariyaratne, H.B.S., Mendis-Handagama, S.M.L.C., Hales, D.B ., Mason, J.I. "Studies on adult Leydig cell precursors and initiation of their differentiation into Leydig cells in the prepurbertal rat testis." Biology of Reproduction 63 165-171 (2000).

Held Hales, K., Diemer, T., Ginde, S., Roberts, M.R., Shankar, B.K., Bosmann, H.B, Hales, D.B. "Diametric effects of bacterial endotoxin lipopolysaccharide on adrenal and Leydig cell Steroidogenic Acute Regulatory Protein (StAR)." Endocrinology 141:4000-4012 (2000).

Cascio, C., Brown, R.C., Hales, D.B ., Papadopoulos, V. "Pathways of dehydro-epiandrosterone formation in developing rat brain glia." Journal of Steroid Biochemistry and Molecular Biology 75: 177-186 (2000)

Fritz S, Grünert RA, Stocco DM, Hales DB , Mayerhofer A "StAR is increased by muscarinic receptor activation in human luteinized granulosa cells." Molecular and Cellular Endocrinology 171:49-51 (2001)

Wang, Z.J., Ito, M., Jeffs, B., Hales, D.B ., Yu, R.N., Jameson, J.L. " Aromatase Expression is Upregulated by Targeted Disruption of Dax-1" Proceedings National Academy of Sciences, USA 98(14):7988-7993 (2001)

Artemenko, I.P., Zhao, D., Hales, D.B. , Held Hales, K., Jefcoate, C.R. "Mitochondrial processing of newly synthesized StAR, but not total StAR, mediates cholesterol transfer to P450scc in adrenal cells." Journal of Biological Chemistry 276: 46583-46596 ( 2001)

Shea-Eaton, W., Sandhoff, T.W., Lopez, D., Hales, D.B. , McLean, M.P. "Transcriptional Repression of the Rat Steroidogenic Acute Regulatory (StAR) Protein Gene by the AP-1 Family Member c-Fos." Molecular Cellular Endocrinology 188:161-170 (2002)

Moons, D., Jirawatnotai, S., Tsutsui, T., Hales, D.B ., Gibori, G., Fazleabas, AT, Kiyokawa, H. " Luteal differentiation is impaired and uncoupled with ovulation in mice deficient for cyclin-dependent kinase-4 (CDK4)" Endocrinology 143: 647-654 (2002)

Sorianello, E., Fritz, S., Beyer, C ., Hales, D.B., Mayerhofer, A., Libertun, C., Lux-Lantos, V. "Development Of An Experimental Ovarian Tumor: Immunocytochemical Analysis" European Journal of Endocrinology 147:387-395 (2002)

Salvador, L.M., Maizels, E., Miyamoto, E., Yamamoto, H., Hales, D.B ., Hunzicker-Dunn, M. "Luteinizing Hormone (LH)-Induced Phosphorylation of p42/44 Mitogen-Activated Protein Kinase (p42/44 MAPK) is Dependent on Protein Kinase A (PKA)." Endocrinology 143: 2986-2994 (2002).

Hales, D.B. " Testicular macrophage modulation of Leydig cell steroidogenesis" Journal of Reproductive Immunology 57:3-18 (2002)

Granot, Z., Silverman, E., Friedlander, R., Melamed-Book, N., Eimerl, S., Timberg, R., Held Hales, K., Hales, D.B ., Stocco, D.M., Orly, J. "The life cyle of the steroidogenic acute regulatory protein (StAR): from transcription through proteolysis." Endocrine Research 28(4): 375-386 (2002).

Diemer, T., Hales, D.B., Weidner. W. "Immune-endocrine interactions and Leydig cell function: the role of cytokines." Andrologia 35:55-63 (2003)

Jirawatnotai, S., Moons. D.S., Stocco, C.S., Franks, R., Hales, D.B. , Gibori, G., Kiyokawa, H. " The CDK Inhibitors p27 Kip1 and p21 Cip1 Cooperate to Restrict Proliferative Life Span in Differentiating Ovarian Cells " Journal of Biological Chemistry 278:17021-7 (2003)

Diemer, T., Allen, J.A., Held Hales, K., Hales, D.B. " Reactive Oxygen Disrupts Mitochondria in MA-10 Tumor Leydig cells and Inhibits Steroidogenic Acute Regulatory (StAR) Protein and Steroidogenesis" Endocrinology 144:2882-91 (2003)

Sierra, A., Lavaque, E., Perez-Martin, M., Azcoitia, I., Hales, D.B. , Garcia-Segura, L.M. " Steroidogenic Acute Regulatory Protein In The Rat Brain: Cellular Distribution, Developmental Regulation And Overexpression After Injury" European Journal of Neuroscience , 18(6): 1458-1467 (2003).

Provost, A., Pequignot, M., Sainton, K., Gadin, S., Salle, S., Marchant, D., Hales, D.B ., Abitbol, M. Expression of SR-BI receptor and StAR protein in rat ocular tissues. C R Biol 326:841-51 (2003)

Granot, Z., Silverman, E., Friedlander, R., Melamed-Book, N., Eimerl, S., Timberg, R., Held Hales, K., Hales, D.B ., Stocco, D.M., Orly, J. "Proteolysis of normal and mutated steroidogenic acute regulatory (StAR) proteins in the mitochondria: the fate of unwanted proteins." Molecular Endocrinology 17: 2461-2476 (2003)

Benmessahel, Y., Troadec, J., Cadepond, F., Guennoun, R., Hales, D.B ., Schumacher, M., Groyer, G. "Down-regulation of Steroidogenic Acute Regulatory (StAR) gene expression by cAMP in cultured Schwann cells " Glia 45:213-228 (2004)

Slevage, D. Hales, D.B ., Rivier, C. "Comparision between the influence of systemic and central injection of alcohol on Leydig cell activity." Alcoholism: Clincal and Experimental Research 28(3): 480-8 (2004)

LeHoux, J. G., A. Fleury, Hales, D.B . "Phosphorylation of the hamster adrenal steroidogenic acute regulatory protein as analyzed by two-dimensional polyacrylamide gel electrophoreses." Molecular Cellular Endocrinology 215(1-2): 127-34 (2004 )

Payne, A.H., Hales, D.B . " Overview of Steroidogenic Enzymes in the Pathway from Cholesterol to Active Steroid Hormones" Endocrine Reviews 25:947-970 (2004).

Allen, J.A., Diemer, T., Held Hales, K., Janus, P., Hales, D.B. " Bacterial Endotoxin Lipopolysaccharide and Reactive Oxygen Species Inhibit Leydig Cell Steroidogenesis via Perturbation of Mitochondria" Endocrine, 25: 265-276 (2004)

Hauet, T., Yao, Z-X., Bose, H.S., Wall, C.T., Han, Z., Li,W., Hales, D.B. , Miller, W.L., Culty, M., Papadopoulos, V. "Peripheral-Type Benzodiazepine Receptor-Mediated Action of Steroidogenic Acute Regulatory Protein on Cholesterol Entry Into Leydig Cell Mitochondria" Molecular Endocrinology 19(2):540-554 (2005)

Hales, D.B ., Allen, J.A., Shankara, T.C., Janus, P., Buck, S., Diemer, T., Held Hales, K. "Mitochondrial Function In Leydig Cell Steroidogenesis" New York Academy of Sciences (in press, 2005)

Allen, J.A., Shankara, T.C., Janus, P., Buck, S., Diemer, T., Held Hales, K. Hales, D.B "The mitochondrial membrane potential ( D Y m), hydrogen ion gradient (?pH) and ATP synthesis are required for Leydig cell steroidogenesis" Endocrinology 147 3924-3935 (2006)

Stammer, K., Edassery, S.L., Barua, A., Bitterman, P., Bahr, J.M., Hales, D.B., Luborsky, J. “Selenium-Binding Protein 1 expression in ovaries and ovarian tumors of the laying hen, a spontaneous model of human ovarian cancer.”  Gynecologic Oncology 109(1):115-21 (2008)

Hales, D.B, Zhuge, Y., Lagman, JA, Ansenberger, K, Mahon, C, Barua, A., Luborsky, J., Bahr, JM. “Cyclooxygenase Expression and Distribution in the Normal Ovary and Their Role in Ovarian Cancer in Gallus Domesticus” Endocrine  33:235-24(4 2008)

Barua, A., Edassary, S.L., Bitterman, P., Abramowicz, J.S., Dirks, A., Bahr, J.M., Hales, D.B., Bradaric, M.J., Luborsky, J.L. “Prevalence of anti-tumor antibodies in laying hen model of human ovarian cancer” Int  J Gyn Cancer 19(4):500-507 (2009)

Barua A, Bitterman P, Abramowicz J, Bradaric MJ, Edassery SL, Dirks A, Hales DB, Bahr JM and Luborsky JL, (2008) Histopathology of ovarian tumors in laying hens, a preclinical model of human ovarian cancer. International Journal of Gynecological Cancer: 19(4):531-539 (2009)

Zhuge, Y., Ansenberger, K., Mahon, C., Lagman, JA. J., Bahr, J., Hales, D.B. “Cyp1B1 Expression in Ovarian Cancer in the Laying Hen Gallus Dometicus.” Gynecological Oncology 112 (1):171-178 (2009)

Attar, E., Tokunaga, H., Imir, G., Yilmaz, M.B., Redwine, D.,Putman, M., Gurates, B., Attar, R., Yaegashi, N., Hales, D.B., Bulun, S.E. “Prostaglandin E2 Coordinately Regulates Transcription of Steroidogenic Genes Necessary for Estrogen Synthesis in Endometriosis”  Journal of Clinical Endocrinology and Metabolism. 94(2): 623-31 (2009)

Barua A, Abramowicz JS, Bitterman P, Bahr JM, Hales DB, Luborsky JL. OP17.08: Transvaginal ultrasound predicts ovarian tumor associated neo-angiogenesis. Ultrasound Obstet Gynecol; 32:370 (2008)

Locke, J., Fazli, L., Adomat, H., Smyl, J., Weins, K., Barr, S., Lubik, A., Hales, D.B., Nelson, C., Gleave, M., Guns, E. “A novel communication role for CYP17A1 in the progression of castration-resistant prostate cancer” The Prostate 69(9): 928-37 (2009)

Ansenberger, K., Zhuge, Y., Lagman, J.A., Richards, C., Barua, A., Bahr, J.M., Hales, D.B., “E-cadherin Expression in Ovarian Cancer in the  Laying Hen, Gallus Domesticus, compared to Human Ovarian Cancer” Gynecologic Oncology 113: 362-369(2009)

Barua, A., Bitterman,P., Bahr, J.M., Bradaric, M., Hales, D.B. Luborsky, J., Abramowicz, J. "Detection of tumor associated neo-angiogenesis by Doppler ultrasound during early stage ovarian cancer in laying hens: A preclinical model of human spontaneous ovarian cancer" American Journal of Ultrasound in Medicine (JUM) 29:173–182 (2010)

Ansenberger, K., Richards, C., Barua, A., Bahr, J.M., Luborsky, J.L., Hales, D.B.  “Decreased severity of ovarian cancer and increased survival in hens fed a flaxseed enriched diet for one year”  Gynecological Oncology 117:341-347 (2010)

Manuscripts in preparation

Zhuge, Y., Tuan, K., Richards, C, Ansenberger, K., Rui, Y., van Breemen, R.B., Bahr, J.M., Hales, D.B., “The effect of a flaxseed enriched diet on cyclooxygenases in ovarian cancer in the laying hen Gallus Domesitcus” manuscript in preparation

Breen, H., Ekewana, O., Dothager, C., Pu, Y.B., Prins, G, Hales,D.B. "Neonatal estrogen causes latent induction of CYP1B1 in adult ventral prostate of rats: role in oxidative stress and neoplasitic transformation" Manuscript in preparation

Nardulli, B.A., Culhane, J., Held Hales, K., Hales, D.B . " The Cyclic Adenosine 3', 5' Monophosphate Mediated Transcriptional Regulation of The Murine Cyp17 Gene" Manuscript in preparation

Diemer, T., Held Hales K, Choi., J., Rommerts, F.F.G. Hales, D.B. "Effect of Ethane 1,2-Dimethane Sulfonate (EDS) on Steroidogenic Acute Regulatory Protein (StAR) Gene Expression, Progesterone Synthesis, and Induction of Apoptosis in cAMP-stimulated Mouse Tumor Leydig cells." Manuscript in preparation

Held Hales, K., Shankar, B., Rivier, C. Hales, D.B. "Interleukin-6 (Il-6) inhibits cAMP-stimulated testosterone primarily by blocking P450c17 transcription in mouse Leydig cells." Manuscript in preparation.

Chapters in Books

Payne, A.H., Anakwe, O.O., Hales, D.B. , Georgiou, M., Perkins, L.M., and Quinn, P.G. "Regulation of Mitochondrial and Microsomal Cytochrome P-450 enzymes in Leydig cells." In Regulation of Ovarian and Testicular Function (Mahesh, V.B., Dhindsa, D.S., Anderson, E., Kalra, S.P., eds.) Plenum Press, N.Y., pp. 467-487, 1987.

Hales, D.B . "Macrophage-Leydig Cell Interactions: An Overview" in The Leydig Cell (Payne, A.H., Hardy, M.P., Russell, L.D. eds.). Cache River Press, Vienna, IL, pp. 451-466, 1996.

Hales, D.B ."Cytokines and Testicular Function" in Cytokines and Reproduction (Hill, J.A. ed.) John Wiley & Sons, NY, NY. pp. 17-42, 2000.

Hedger, M.P., Hales, D.B "Reproductive Immunology of the Male" in Physiology of Reproduction (Neil, J. ed ) Elsevier, San Diego (3 rd edition) Chapter 25, pp:1195-1286, 2006.

Invited Papers

Hales, D.B. "Editorial: Gonadal-Specific Transcription Factors-GATA (Go) 4 It!" Endocrinology 124:974-976 (2001)

Hales, D.B. "Editorial: Another piece in the maddening puzzle of declining steroidogenesis in aging Leydig cells." Journal of Andrology, 23:327-329 (2002)

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updated: 04/28/2010 10:43 AM