MBMB 451b

Copyright 2000, E.C. Niederhoffer

Sample Problem

Glutathione reductase is an enzyme that is responsible for maintaining the reduced form of glutathione (GSH) in the serum. Briefly explain the key features of this enzyme and the flow of electrons that occurs during the catalytic steps.

Key features:

Catalytic steps:

Formation of SH---FAD charge-transfer complex and H-bonding network between Glu-His-Cys.
NADP+ is released
One GSH released by protonation by His (general acid)
Cys63 attacks mixed disulfide to release 2nd GSH and regenrate redox-active disulfide

 

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