Problem Unit #4

Copyright 2000, E.C. Niederhoffer

Lecture #6

You can click on the link to listen to a RealAudio file of the day's lecture. The following description contains the material discussed and the source of the overheads used during the presentation. You can click on the linked ECN or CC files while listening to the audio.

Code:

D = Devlin (Textbook of Biochemistry with Clinical Correlations)

ECN = Created by me

MV = Mathews & van Holde (Biochemistry)

VV = Voet & Voet (Biochemistry)

Lecture 6 (1/4/00) 51 minutes

Announcements

• Remember to check the Web site for the latest updates and corrections
• Answers to the Pacing Quiz are posted here and on the PU04 home page
• Final examination (30 short-answer questions/problems) is scheduled for 8-10 am on Tuesday 18 January 2000 in LS I Rm 133

Lecture #5 review (D Fig 8.03)

• Pentose phosphate pathway, tissue localization, NADPH (glutathione), ribose-5-phosphate (nucleotides), glycolytic intermediates
• Regulation by NADP⁺ (oxidative phase), relationship to fatty acid synthesis

Sugar interconversions and nucleotide sugar formation

• Isomerization, phosphorylation, epimerization, decarboxylation, redox, transamidation reactions (D Figs 8.04a, 8.04b)
• Use of nucleotide-linked sugar intermediates (D Figs 8.04a, 8.04b)
• Glucuronic acid synthesis and degradation, regulation by UDP-xylose (D Figs 8.05, 8.06, 8.07a, 8.07b, 8.07c)
• Sialic acid synthesis, use of CTP (D Fig 8.08)

Biosynthesis of complex carbohydrates

• Glycosyltransferases (ECN)

Glycoproteins

• Overview (ECN)
• O- and N-linked glycoproteins, core structure, lipid-linked (dolichol phosphate), enzymic defects (D Figs 8.09, 8.10, CC 8.7)

Proteoglycans

• Overview (ECN)
• Six classes (hyaluronate, chondroitin sulfate, dermatan sulfate, heparin, heparan sulfate, keratan sulfate), enzymic defects (D Fig 8.11, CC 8.9)
• Synthesis of chondroitin sulfate, synthesis of 3'-phosphoadenosine-5'-phosphosulfate (D Fig 8.12, 8.13)

Preview of lecture #7

• Pyruvate dehydrogenase complex, pyruvate to acetyl-CoA (D Fig 6.15)

Remember to look over the Study Guide and textbook sections for additional illustrations that may assist in your understanding of the presented material.

PU04 Home Page